There are some reports on effects of vibration frequency, acceleration, and room temperature on temporary threshold shift (TTS) of fingertip vibration sensation. Handle temperature seems to affect TTS of fingertip vibration sensation. The aim of the present study was to investigate the effect of different handle temperatures on TTS of fingertip vibration sensation. Six healthy male subjects were exposed to acute vibration with frequency at 125 Hz and acceleration at 46 m/s 2 (rms) at different handle temperatures of 10, 20 or 30 °C for 0.5, 1, 3, 5 or 10 min in a temperature controlled room at 24 ± 1 °C. Immediately after vibration exposure or handle gripping only, the fingertip vibration sensation threshold at 125 Hz was measured at the tip of the right-middle finger. The TTS was larger at lower handle temperature after acute vibration exposure of same duration. After gripping of the handle at lower temperature, the TTS was larger with longer gripping durations. The FST decreased when subjects were gripping handle with or without vibration exposure with 3, 5 or 10 min of gripping time. From the results, it can be concluded that TTS is large at lower handle temperature and is larger with acute vibration exposure followed by the situation with longer handle gripping time.