Serum from injured automobile (AUT) and motorcycle (MTC) drivers treated at a trauma center was tested for d-9-tetrahydrocanna-binol (THC) activity to determine pre-crash marijuana use. From June 1990 to March 1991, samples from approximately 20 AUT drivers per month and all MTC drivers were available for testing. Also, toxicology screens were performed for ethyl alcohol (ETOH), cocaine (CO), and phencyclidine (PCP) among the driver groups. Six (2.7%) of the 225 AUT drivers and 34 (32.0%) of the 106 MTC drivers were THC+ (P<0.001). Compared with a prior study, the THC+ rate decreased significantly from 31.8% among AUT drivers (P<0.001) but had not changed significantly from the 38.6% rate among MTC drivers. Positive toxicology rates were higher among the 1077 AUT and 261 MTC drivers tested for ETOH, CO, and PCP, being 35.2% vs 47.1% (P<0.001), 5.0% vs 8.0% (P<0.08), 1.5% vs 3.1% (NS), respectively.