This study evaluated the differences between daytime and nighttime driving performances of selected individual drivers. Forty subjects, evenly divided by sex, of similar age and driving experience, were selected from students attending a midwestern university. All subjects were tested by driving the same test vehicle over the same test routes two times, once in the daytime and once at nighttime. The test vehicle measured fine and coarse steering reversals, brake applications, and average speed in one-minute intervals over each of three route sections. The findings of this study indicated that there were significant differences in driving performance from daytime to nighttime between all subjects (male and female). There were no significant differences in driving performance between subjects in different driving orders. Each route section seemed to make a difference in driving performance during both daytime and nighttime driving. Implications are made to a driver education setting.