Blunt arterial injury experimentally occurred in distraction with intimal subfailure before ultimate failure. Experimental soft-tissues are commonly frozen until testing and may be refrigerated between tests. The present study axially distracted porcine aortas to failure implementing fresh, refrigerated, and frozen storage conditions. Intimal subfailure occurred in 83% of specimens. Elasticity and subfailure and failure stresses decreased in refrigerated compared to fresh and frozen specimens. Fresh and frozen specimens were not significantly different. Clinically relevant intimal failures can be reproduced and injury mechanics determined while adhering to experimental protocols of freezing specimens before testing. However, short-term tissue refrigeration may affect biomechanics.
Keywords:
Carotid artery injury; tunica intima; experimental storage techniques; subfailure