The current study evaluates the Total Human Model for Safety (THUMS) AM50 v6.1 and the Global Human Body Model Consortium (GHBMC) M50-O v6.0 in moderate-speed rear impact (ΔV of 24 km/h) and compares model head, cervical spine, and T1 kinematics to previously published PMHS data. An open-source finite element (FE) seat model was extracted from the 2012 Toyota Camry FE model developed by George Mason University. Sled acceleration data obtained during testing were used to drive simulated sled motion, and occupant kinematics were recorded. Normalised Root Mean Square Deviation (NRMSD) and cross-correlation methods outlined in ISO18571 were used to quantify differences in time-history data between PMHS and HBMs. The desired 10% threshold for average NRMSD was not met by either HBM for any of the response data evaluated. Average ISO rating showed fair biofidelity (THUMS = 0.62, GHBMC = 0.62) for both model response data compared to the two PMHS data sets available. Peak intervertebral rotations in the cervical spine trended similarly in both HBMs, with the best agreement in the upper levels. Study outcomes contribute to the understanding of the biofidelity of the head-neck responses of the HBMs seated on a production seat and subjected to moderate-speed rear impact.