Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections

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Junqueira, L. C. U.¹; Bignolas, G.¹; Brentani, R. R.¹

Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections

Histchem J. 1979;11:447-455

©1979 Chapman and Hall Ltd

Affiliations

¹Laboratórios de Biologia Celular, Pesquisa em Reumatologia e Oncologia Experimental, Faeuldade de Medicina, Universidade de São Paulo, Brazil
Abstract

Sirius Red, a strong anionic dye, stains collagen by reacting, via its sulphonic acid groups, with basic groups present in the collagen molecule. The elongated dye molecules are attached to the collagen fibre in such a way that their long axes are parallel. This parallel relationship between dye and collagen results in an enhanced birefringency.

Examination of tissue sections from 15 species of vertebrates suggests that staining with Sirius Red, when combined with enhancement of birefringency, may be considered specific for collagen. An improved and modified method of staining with Sirius Red is presented.
Links

DOI: 10.1007/BF01002772

PubMed: 91593

WoS: A1979HG03900007
History

Received: 1978-12-11

Revised: 1979-03-02

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