Introduction:​ While bone literature abounds with correlations of mechanical stiffness to mineralization, such correlations are reported without relating the findings to specific intracortical locations. This study reports on mapping of stiffness and mineralization distributions in ring-shaped cortical bone samples sliced from mid-diaphyseal bovine femur. Stiffness and mineralization measurements were conducted at points across the intracortical thickness along radial lines emanating from the inner (endosteal) surface to the outer (periosteal) surface. Measurements were taken along approximately 4 mm distance of cortical bone thickness.

Materials and methods:​ Three experimental techniques were employed:​ Vickers microhardness (HV), energy-dispersive X-ray (EDX) spectroscopy, and computed tomography (CT). Stiffness values were extracted from the Vickers microhardness tests. Elemental mineralization values (calcium %wt. and phosphorus %wt.) were determined from EDX data. All measurements were repeated on three different femur bones taken from different bovines (collected fresh from butcher).

Results:​ The study plots stiffness values and elemental mineralization (calcium %wt. and phosphorus %wt.) versus cortical thickness. Both stiffness and Ca %wt. and P %wt. are found to track and to linearly increase when plotted along the radial distance. The stiffness and mineralization trends collected from Vickers and EDX measurements were verified by employing the CT number (Hounsfield units, HU) via CT scans of the same bone samples. Data fitting via statistical methods revealed that all correlations were statistically significant.

Conclusion:​ Starting from endosteal to periosteal surfaces of mid-diaphyseal bovine femur, it was found that stiffness, mineralization, and HU values all exhibit increasing and correlating trends.