The need to quantify and understand the mechanical properties of human abdominal organs is becoming increasingly important in the automotive industry because of the increased incidence and severity of injuries to these organs in motor vehicle crashes. Because of how quickly these tissues degrade after death, the need to develop appropriate procurement and testing methodology is imperative. The purpose of this paper was to collect data from uniaxial tension tests to determine the effects of freezing on the mechanical properties of bovine liver parenchyma. One fresh, never frozen bovine liver was divided in half with one half being frozen and the other half tested immediately. Each half was sliced and stamped so that multiple tension coupons were produced. Each specimen was then pulled to failure. A total of 8 tests were run on the fresh half and a total of 8 tests were run on the frozen half. The fresh and frozen average failure stress values were not statistically different, p>0.05;​ however, the frozen half’s average failure strain was 49% less than that of the fresh half’s average failure strain, p<0.001. The difference in the average failure strains between the two halves shows that to determine the material properties of internal organ tissue the organs can not be frozen prior to testing.