Controlled growth factor release from synthetic extracellular matrices

Lee, Kuen Yong; Peters, Martin C.; Anderson, Kenneth W.; Mooney, David J.

Affiliations

¹Department of Biologic & Materials Sciences, University of Michigan, Ann Arbor, Michigan 48109, USA

²Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA

³Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA

⁴Department of Otolaryngology & Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan 48109, USA

Abstract

Polymeric matrices can be used to grow new tissues and organs^1,2, and the delivery of growth factors from these matrices is one method to regenerate tissues^3,4. A problem with engineering tissues that exist in a mechanically dynamic environment, such as bone, muscle and blood vessels^5,6, is that most drug delivery systems have been designed to operate under static conditions. We thought that polymeric matrices, which release growth factors in response to mechanical signals, might provide a new approach to guide tissue formation in mechanically stressed environments. Critical design features for this type of system include the ability to undergo repeated deformation, and a reversible binding of the protein growth factors to polymeric matrices to allow for responses to repeated stimuli. Here we report a model delivery system that can respond to mechanical signalling and upregulate the release of a growth factor to promote blood vessel formation. This approach may find a number of applications, including regeneration and engineering of new tissues and more general drug-delivery applications

Links

DOI: 10.1038/35050141

PubMed: 11140690

WoS: 000165951100056

History

Received: 2000-05-11

Accepted: 2000-09-25

Cited Works (1)

Year	Entry
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Cited By (11)

Year	Entry
2003	Drury JL, Mooney DJ. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials. November 2003;24(24):4337-4351.
2010	Zhu J. Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering. Biomaterials. June 2010;31(17):4639-4656.
2011	Kolambkar YM, Dupont KM, Boerckel JD, Huebsch N, Mooney DJ, Hutmacher DW, Guldberg RE. An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects. Biomaterials. January 2011;32(1):65-74.
2011	Kolambkar YM, Boerckel JD, Dupont KM, Bajin M, Huebsch N, Mooney DJ, Hutmacher DW, Guldberg RE. Spatiotemporal delivery of bone morphogenetic protein enhances functional repair of segmental bone defects. Bone. September 2011;49(4):485-492.
2016	McDermott AM, Mason DE, Lin AS, Guldberg RE, Boerckel JD. Influence of structural load-bearing scaffolds on mechanical load- and BMP-2-mediated bone regeneration. J Mech Behav Biomed Mater. September 2016;62:169-181.
2011	Boerckel JD, Uhrig BA, Willett NJ, Huebsch N, Guldberg RE. Mechanical regulation of vascular growth and tissue regeneration in vivo. Proc Natl Acad Sci USA. September 13, 2011;108(37):E674-E680.
2009	Kolambkar YM. Electrospun Nanofiber Meshes for the Functional Repair of Bone Defects [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; December 2009.
2015	Priddy LB. Biomaterial Strategies for Improved Bone Healing With Bone Morphogenetic Protein-2 Delivery [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; August 2015.
2019	McDermott AM. Mechanical Regulation of Progenitor Cell Differentiation During Endochondral Ossification [PhD thesis]. Notre Dame, IN: University of Notre Dame; July 2019.
2018	Sawyer SW. Structurally Supported Cell-Laden Scaffolds for Bone Tissue Regeneration [PhD thesis]. University of Syracuse; December 2018.
2005	Albrecht DR. Microscale Organization of Cells Within 3-D Hydrogels by Dielectrophoresis: Applications to Cartilage Biology [PhD thesis]. San Diego (UCSD), University of California; 2005.