Although evidence suggests that yield strains for trabecular bone are isotropic, i.e., independent of loading direction, decisive support for this hypothesis has remained elusive. To explicitly test whether yield strains for trabecular bone are isotropic, compressive and tensile yield strains of 51 specimens of bovine tibial trabecular bone (0.41 ± 0.08 g/cm³ [mean apparent density ± SD]) were measured without end artifacts in on-axis (along the principal trabecular orientation) and off-axis (30–40° oblique to on-axis) orientations. Yield strains for the on-axis and off-axis orientations were similar in tension (0.80 ± 0.03% compared with 0.85 ± 0.04%, p = 0.21) and compression (0.97 ± 0.05% compared with 0.96 ± 0.07%, p > 0.99); as expected, modulus and strength depended on loading direction. When considered with an ancillary experiment on bovine tibial trabecular bone that showed yield strains to be similar between on-axis and 90° off-axis bone, these results firmly establish the isotropy of uniaxial yield strains for bovine tibial trabecular bone. This bone is of high density and has a strong, plate-type, anisotropic architecture. Therefore, yield strains for uniaxial loading are expected to be isotropic, or nearly so, for other types of dense trabecular bone, although further work is required to confirm this and to establish this behavior for bone of lower density.